Invemar
Bulletin of Marine and Coastal Research

A NON-INVASIVE METHOD FOR EXTRACTING DNA FROM CORYPHAENA HIPPURUS

Bruna Rafaela Caetano Nunes Pazdiora, John Josephraj Selvaraj, Diego Fernando Marmolejo, Jaime Eduardo Muñoz, Ángela Inés Guzmán

Abstract


A protocol for DNA extraction and effect of the type of tissue material for obtaining DNA from dolphinfih in high quantity and quality for molecular studies was evaluated. The DNA of 25 individuals was extracted from the digestion of the muscle and the dorsal fi, with proteinase K, and the extraction kit for blood and animal tissue. DNA were obtained in all the samples; however, dorsal fis samples showed signifiantly higher amount of DNA than muscle samples. The defiition of an appropriate methodology of DNA extraction can contribute to studies of genetic structure of the species. DNA extraction from dolphinfih dorsal fis, non-invasive method, is an alternative that is environmentally, socially, and commercially viable, where sample collection does not require the sacrifie of the animal and do not alter its economic value.

Keywords


Dorsal fi; DNA; dolphinfih; Colombian Pacifi

Full Text: PDF

DOI: 10.25268/bimc.invemar.2015.44.2.33

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