Bulletin of Marine and Coastal Research

Vol. 52 No. 1 (2023)
Research Articles

In vitro and in vivo inhibition of Vibrio spp. isolated from the shrimp Litopenaeus vannamei using native lactic acid bacteria

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Vicsy Yanet Tinoco Elizalde
Universidad Nacional de Tumbes
Tessy Peralta Ortiz
Universidad Nacional de Tumbes
Bio
Alberto Ordinola Zapata
Universidad Nacional de Tumbes (Perú)
Bio
DOI: https://doi.org/10.25268/bimc.invemar.2023.52.1.1156

Published 2023-06-15

Keywords

  • shrimp culture,
  • vibriosis,
  • bacterial resistance,
  • probiotic,
  • alternative to antibiotics

How to Cite

1.
Tinoco Elizalde VY, Peralta Ortiz T, Ordinola Zapata A. In vitro and in vivo inhibition of Vibrio spp. isolated from the shrimp Litopenaeus vannamei using native lactic acid bacteria. Bol. Investig. Mar. Costeras [Internet]. 2023 Jun. 15 [cited 2025 Mar. 6];52(1):9-26. Available from: https://boletin.invemar.org.co/ojs/index.php/boletin/article/view/1156

Copyright (c) 2023 Vicsy Yanet Tinoco Elizalde, Tessy Peralta-Ortiz, Alberto Ordinola-Zapata

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Abstract

The diseases caused by Vibrio spp. cause the greatest economic losses in shrimp farming, antibiotic therapy has led to the emergence of resistant strains and research is being carried out into the use of probiotics for their treatment. This research aimed to obtain native lactic acid bacteria (LAB) isolated from cultured shrimp and to determine their probiotic potential against antibiotic
resistant Vibrio spp in vitro and in vivo. Nine strains of both LAB and Vibrio spp. were isolated from cultured shrimps from Tumbes. Antibiotic–resistance of Vibrio spp. was determined; as well as the in vitro inhibitory power of LAB strains against these strains by agar well trial and in vivo, by experimental infection of shrimp with Vibrio spp. strains and treatment for eight days with LAB strains added
to feed. The nine strains of Vibrio spp. isolated from cultured shrimps were resistant to fosfomycin and nalidixic acid, with a multiple antibiotic resistance index (MAR) of 0.2. In the in vitro trial, the C1L, F1L and F3L strains inhibited the largest number of Vibrio spp. strains; also in vivo, the C1L and F1L strains significantly (p < 0.05) increased survival and decreased the Vibrio spp. count of infected
shrimp in the hepatopancreas. It is concluded that antibiotic- resistant strains of Vibrio spp. isolated from cultured shrimps could be inhibited by native LAB strains, C1L and F1L, demonstrating a promising probiotic potential.

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