Insights into the Genetic Diversity of the leopard seal (Hydrurga leptonyx), inferred from mitochondrial DNA analysis, at Danco Coast, Antarctic Peninsula
DOI:
https://doi.org/10.25268/bimc.invemar.2021.50.SuplEsp.0951Keywords:
Leopard seal, pinnipeds, genetic diversity, Control Region, Antarctica.Abstract
The leopard seal (Hydrurga leptonyx) has a wide distribution throughout the Antarctic circumpolar region. This species has solitary habits, haul out on pack ice for long period, and individuals tend to congregate in the same areas during the mating season. The leopard seal shows site fidelity to reproductive sites, which may imply restricted gene flow between groups. In order to conduct the first assessment of genetic diversity of leopard seals in waters around Danco Coast in the Antarctic Peninsula, we amplified mitochondrial DNA Control Region (mtDNA-CR, 423 bp) from DNA extracted from 13 blood samples collected in Primavera Base (62º15'S, 58º39'W) during 2011-2012 austral summer. Our results showed high haplotype diversity (h = 0.989), represented in one unique haplotype for each sample, and only one haplotype shared between two samples. Network analyses confirmed high diversity with some divergent haplotypes. Our genetic findings suggest that individuals sampled belong to one population. Due to ice dynamic as a consequence of climate change, many individuals are shifting their diet habits, showing dietary specializations that may have the potential to promote genetic differentiation in the long-term. However, given the lack of leopard seals genetic studies, this hypothesis cannot be tested. Research studies using mitochondrial and nuclear markers are needed to assess the genetic status of leopard seals and their population structure in relation with other populations, particularly to determine restrictions to gene flow likely related to dietary specialization among leopard seals populations.
References
Bandelt, H.J., P. Forster y A. Röhl. 1999. Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol., 16: 37–48.
Barragán-Barrera, D.C., L. J. May-Collado, G. Tezanos-Pinto, V. Islas-Villanueva, C. Correa-Cárdenas, C.A. y S. Caballero. 2017. High genetic structure and low mitochondrial diversity in bottlenose dolphins of the archipelago of Bocas del Toro, Panama: a population at risk? PLoS One, 12(12): e0189370.
Bonillas-Monje, M. 2018. Historical dynamics of Leopard Seal (Hydrurga leptonyx) and Southern Elephant Seal (Mirounga leonina) populations in the Southern Ocean. Master thesis, Durham University, Durham. Abstract.
Botta, S., E. R. Secchi, T. L. Rogers, J. H. F. Pradoa, R. C. de Lima, P. Carlini y J. Negrete. 2018. Isotopic niche overlap and partition among three Antarctic seals from the Western Antarctic Peninsula. Deep-Sea Res. Pt. II., 149: 240–249.
Casaux, R., A. Baroni, A. Ramón, A. Carlini, M. Bertolin y C. Y. DiPrinzio. 2009. Diet of the leopard seal Hydrurga leptonyx at the Danco Coast, Antarctic Peninsula. Polar Biol., 32(2): 307–310.
Coltman, D. W., G. Stenson, M. O. Hammill, T. Haug, C. S. Davis y T. L. Fulton. 2007. Panmictic population structure in the hooded seal (Cystophora cristata). Mol. Ecol., 16(8): 1639–1648.
Davis, C. S., I. A. N. Stirling, C. Strobeck y D. W. Coltman. 2008. Population structure of ice‐breeding seals. Mol. Ecol., 17(13): 3078–3094.
Drummond, A. J., B. Ashton, M. Cheung, J. Heled, M. Kearse, R. Moir, S. Stones-Havas, T. Thierer y A. Wilson. 2009. Geneious Pro. Version 4.8.5., ver. http://www.geneious.com.
Ducklow, H.W, K. Baker, D. G. Martinson, L. B. Quetin, R. M. Ross, R. C. Smith, S. E. Stammeriohn, M. Vernet y W. Fraser. 2007. Marine pelagic ecosystems: The West Antarctic Peninsula, Philos. Trans. R. Soc. Lond. B., 362: 67−94.
Excoffier, L. y H. E. L. Lischer. 2010. Arlequin suite version 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Res., 10: 564–567.
Forcada, J., P. N. Trathan, K. Reid y E. J. Murphy. 2005. The effects of global climate variability in pup production of Antarctic fur seals. Ecology, 86(9): 2408–2417.
Fyler, C. A., T. W. Reeder, A. Berta, G. Antonelis, A. Aguilar y E. Androukaki. 2005. Historical biogeography and phylogeny of monachine seals (Pinnipedia: Phocidae) based on mitochondrial and nuclear DNA data. J. Biogeogr., 32(7): 1267–1279.
Goudet, J. 2005. HIERFSTAT, a package for R to compute and test hierarchical F-statistics. Mol. Ecol. Res., 5(1): 184–186.
Giraldo, A., M. I. Críales-Hernández, M. Jeréz-Guerrero and D. F. Mojica-Moncada. 2019. Mesozooplankton biomass and epipelagic copepod assemblages in the Gerlache Strait (Antarctica) during the 2015 austral summer. Bol. Invest. Mar. Cost., 48(1): 9–26.
Goldsworthy, S., J. Francis, D. Boness y R. Fleischer. 2000. Variation in the mitochondrial control region in the Juan Fernandez fur seal (Arctocephalus philippii). J. Hered., 91(5): 371–377.
Guerrero, A., J. Negrete, M. Márquez, J. Menucci, K. Zaman y T. Rogers. 2014. Fatty acid composition suggests leopard seals are no longer apex predators in the Western Antarctic Peninsula ecosystem. XXXIII Scientific Committee on Antarctic Research (SCAR). Open Science Conference. Agosto 2014. Auckland, New Zealand.
Hall-Aspland, S. A. y T. L. Rogers. 2004. Summer diet of leopard seals (Hydrurga leptonyx) in Prydz Bay, Eastern Antarctica. Polar Biol., 27(12): 729–734.
Hall-Aspland, S. A., T. L. Rogers y R. B. Canfield. 2005. Stable carbon and nitrogen isotope analysis reveals seasonal variation in the diet of leopard seals. Mar. Ecol. Prog. Ser., 305: 249–259.
Hall-Aspland, S. A. y T. L. Rogers. 2007. Identification of hairs found in leopard seal (Hydrurga leptonyx) scats. Polar Biol., 30(5): 581.
Hernández-León, S., A. Portillo-Hahnefeld, C. Almeida, P. Bécognée y I. Moreno. 2001. Diel feeding behavior of krill in the Gerlache Strait, Antarctica. Mar. Ecol. Prog. Ser., 223: 235–242.
Hückstädt, L. 2015. Hydrurga leptonyx. The IUCN Red List of Threatened Species 2015: e.T10340A45226422, ver. http://dx.doi.org/10.2305/IUCN.UK.2015-4.RLTS.T10340A45226422.en.
IPCC. 2014. Climate change 2014: Synthesis Report. Contribution of Working Groups I, II and III to the fifth assessment report of the Intergovernmental Panel on Climate Change. En: Pachauri, R. K. y L.A. Meyer (Eds.). IPCC. Geneva, Switzerland. 151 p.
Maddison, D. R. y W. P. Maddison. 2011. MacClade 4: Analysis of phylogeny and character evolution. Version 4.08a., ver. http://macclade.org.
Matthee, C. A., F. Fourie, W. H. Oosthuizen, M. A. Meyer y K. A. Tolley. 2006. Mitochondrial DNA sequence data of the Cape fur seal (Arctocephalus pusillus pusillus) suggest that population numbers may be affected by climatic shifts. Mar. Biol., 148(4): 899−905.
Meade, J., M. B. Ciaglia, D. J. Slip, J. Negrete, M. E. I. Márquez, J Mennucci y T.L. Rogers. 2015. Spatial patterns in activity of leopard seals Hydrurga leptonyx in relation to sea ice. Mar. Ecol. Prog. Ser., 521: 265–275.
Mendes, C. R. B., V. M. Tavano, M. C. Leal, M. S. de Souza, V. Brotas y C. A. E. García. 2013. Shifts in the dominance between diatoms and cryptophytes during three late summers in the Bransfield Strait (Antarctic Peninsula). Polar Biol., 36: 537–547.
Mojica-Moncada, D. F., C. Cárdenas, J. F. Mojica-Moncada, D. Holland y G. Casassa. 2019. Estudio del Glaciar Lange y su impacto por cambio climático en la Bahía de Almirantazgo, Isla Rey Jorge, Antártica durante el verano austral 2018 – 2019. XVIII Seminario Nacional de Ciencias y Tecnologías del Mar. Octubre 2019. Barranquilla, Colombia.
Negrete J., E. A. Depino, P. Carlini, J. G. Galliari, S. Leonardi, M. Bobinac, C. M. Loza, M. E. I. Márquez, J. A. Mennucci y T. Rogers. 2014. Fidelidad al sitio de muda de la foca leopardo (Hydrurga leptonyx) en costa Danco, Península Antártica. XXVII Jornadas Argentinas de Mastozoología. Noviembre 2014. Esquel, Provincia de Chubut, Argentina.
Rogers, T.L. 2007. Age-related differences in the acoustic characteristics of male leopard seals Hydrurga leptonyx. J. Acoust. Soc. Amer. 122: 596–605.
Rogers, T. L. 2013. Leopard seal: Hydrurga leptonyx. 550–552. En: Würsig, B., Perrin, W., B. Würsig y J. G. M. Thewissen (Eds.). Encyclopedia of marine mammals, 2nd Ed. Academic Press. Burlington, USA. 1352 p.
Rogers, T. L. 2018. Leopard seal: Hydrurga leptonyx. 673–674. En: Würsig, B., J.G.M. Thewissen y K. Kovacs (Eds.). Encyclopedia of marine mammals, 3rd Ed. Academic Press. London, UK. 1190 p.
Rogers, T.L. y D.H. Cato. 2002. Individual variation in the acoustical behaviour of the adult male leopard seal, Hydrurga leptonyx. Behaviour. 139: 1267–1286.
Sanger, F. y A. R. Coulson. 1975. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol., 94(3): 441–446.
Slade, R. W., C. Moritz y A. Heideman. 1994. Multiple nuclear-gene phylogenies: application to pinnipeds and comparison with a mitochondrial DNA gene phylogeny. Mol. Biol. Evol., 11(3): 341–356.
Slade, R. W., Moritz, C., A. R. Hoelzel y H. R. Burton. 1998. Molecular population genetics of the southern elephant seal Mirounga leonina. Genetics, 149(4): 1945–1957.
Slip, D. J., K. Green, H. R. Burton, P. J. Mitchell y M. A. R. K. Adams. 1994. Allozyme variation in the leopard seal, Hydrurga leptonyx. J. Aust. Mammal. Soc., 17: 1–5.
Steinberg, D. K., D. G. Martinson y D. P. Costa. 2012. Two decades of pelagic ecology of the Western Antarctic Peninsula. Oceanography, 25: 56−67.
Southwell, C., K. Kerry, P. Ensor, E. J. Woehler y T. Rogers. 2003. The timing of pupping by pack-ice seals in East Antarctica. Polar Biol., 26(10): 648−652.
Thomas, J. A. y C. L. Golladay. 1995. Geographic variation in leopard seal (Hydrurga leonina) underwater vocalizations. 201–222. En: Kastelein, R. A., J.A. Thomas y P. E. Nachtigall (Eds.). Sensory Systems of Aquatic Mammals. De Spil Publishers, Woerden, The Netherlands.
Varela, M., E. Fernandez y P. Serret. 2002. Size-fractionated phytoplankton biomass and primary production in the Gerlache and south Bransfield Straits (Antarctic Peninsula) in Austral summer 1995–1996. Deep-Sea Res. Part II. Top. Stud. Oceanogr., 49(4-5): 749−768.
Vaughan, D., G. Marshall, W. Connolley, C. Parkinson, R. Mulvaney, D. Hodgson, J. C. King, C. J. Pudsey y J. Turner. 2003. Recent rapid regional climate warming on the Antarctic Peninsula, Clim. Chang., 60: 243–274.
Westlake, R. L. y G. M. O'Corry-Crowe. 2002. Macrogeographic structure and patterns of genetic diversity in harbor seals (Phoca vitulina) from Alaska to Japan. J. Mammal., 83(4): 1111–1126.
Wood, S. A., T. R. Frasier, B. A. McLeod, J. R. Gilbert, B. N. White, W. D. Bowen, M. O. Hammill, G. T. Waring y S. Brault. 2011. The genetics of recolonization: an analysis of the stock structure of grey seals (Halichoerus grypus) in the northwest Atlantic. Can. J. Zool., 89(6): 490–497.
Wynen, L. P., S. D. Goldsworthy, C. Guinet, M. N. Bester, I. L. Boyd, I. Gjertz, G. Hofmeyr, R. White y R. Slade. 2000. Post sealing genetic variation and population structure of two species of fur seal (Arctocephalus gazella and A. tropicalis). Mol. Ecol., 9(3): 299–314.
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