Boletín de Investigaciones Marinas y Costeras

Vol. 51 No. 1 (2022)
Scientific Notes

The Cytochrome Oxidase I gene confirms the presence of the nemertine Carcinonemertes conanobrieni, a parasite of the Caribbean spiny lobster (Panulirus argus) in Colombia

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  • Amanda Berben Henríquez,
  • Jaime Gonzalez-Cueto,
  • Angie Colorado,
  • Sigmer Quiroga
Amanda Berben Henríquez
Universidad del Magdalena
Matrix of interspecific and intraspecific genetic distances based on 488 bp sequences of the Cytochrome Oxidase I (COI) gene using the K2P nucleotide substitution evolution model (Kimura, 1980). The sequence generated in this study is highlighted in bold; the rest of the sequences were extracted from the GenBank genetic sequence database of the “National Center for Biotechnology Information” (https://www.ncbi.nlm.nih.gov/).
DOI: https://doi.org/10.25268/bimc.invemar.2022.51.1.1166

Published 2022-06-14

Keywords

  • Carcinonemertidae, COI, Puebloviejo

How to Cite

1.
Berben Henríquez A, Gonzalez-Cueto J, Colorado A, Quiroga S. The Cytochrome Oxidase I gene confirms the presence of the nemertine Carcinonemertes conanobrieni, a parasite of the Caribbean spiny lobster (Panulirus argus) in Colombia. Bol. Investig. Mar. Costeras [Internet]. 2022 Jun. 14 [cited 2025 Apr. 10];51(1):201-6. Available from: https://boletin.invemar.org.co/ojs/index.php/boletin/article/view/1166

Copyright (c) 2022 Amanda Berben Henríquez

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Abstract

Morphological identifications complemented with molecular analysis are important for the delimitation of cryptic species. This document contains annotations on the molecular identification of the parasitic nemertine Carcinonemertes conanobrieni in the Caribbean spiny lobsters Panulirus argus. The collected nemerteans were found parasitizing ovigerous lobsters from the Gulf of Salamanca (Puebloviejo), Colombia. DNA was extracted from a specimen and a fragment of the mitochondrial gene Cytochrome Oxidase I (COI) was amplified using Folmer’s universal primers, the sequence was edited with ProSeq 3.5 and aligned with all the C. conanobrieni sequences available in GenBank. A sequence of 488 base pairs corresponding to the section of the genetic barcode of the COI gene was obtained, the matrix of genetic distances showed little variation when comparing the obtained sequence with those available in the GenBank database. These results confirm the presence of this nemertean in the Panulirus argus lobsters in Colombia providing

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