Bulletin of Marine and Coastal Research

Vol. 53 No. 1 (2024)
Research Articles

Immunotoxicity and lysosomal damage on Pinctada imbricata (Röding) exposed to used automobile crankcase oils

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  • Edgar Alexander Zapata Vivenes,
  • Gabreial Sanchez,
  • Leida Marcano
Edgar Alexander Zapata Vivenes
Escuela de Ciencias, Nucleo de Sucre, Universidad de Oriente
Bio
Gabreial Sanchez
Laboratorio de Bioquímica y Ecotoxicología, Departamento de Biología, Escuela de Ciencias, Nucleo de Sucre, Universidad de Oriente, Venezuela
Leida Marcano
Laboratorio de Bioquímica y Ecotoxicología, Departamento de Biología, Escuela de Ciencias, Nucleo de Sucre, Universidad de Oriente, Venezuela
DOI: https://doi.org/10.25268/bimc.invemar.2024.53.1.1208

Published 2024-01-01

Keywords

  • Hemocyte; lysosomal destabilization; oil; immunology; stress

How to Cite

1.
Zapata Vivenes EA, Sanchez G, Marcano L. Immunotoxicity and lysosomal damage on Pinctada imbricata (Röding) exposed to used automobile crankcase oils. Bol. Investig. Mar. Costeras [Internet]. 2024 Jan. 1 [cited 2025 Jan. 22];53(1):9-24. Available from: https://boletin.invemar.org.co/ojs/index.php/boletin/article/view/1208

Copyright (c) 2023 Edgar Alexander Zapata Vivenes, Gabreial Sanchez, Leida Marcano

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Abstract

The effect of exposure to water-soluble fractions of used automobile crankcase oils (WSF-AUCO) on haematology parameters, immune system, stability of lysosomal membranes of hemocytes and lipid peroxidation in the digestive gland of the pearl oyster Pinctada imbricata was evaluated in this study. The oysters were exposed to 0, 1, 10 and 20 % v/v of WSF-AUCO during different periods: 3, 5 and 7 d, in static aquaria under controlled conditions (oxygenation 6 mg/L; 25 ± 1 °C; pH 8.0 and 36 ‰). During the early period (3d) of exposure, an increase in the total count of hemocytes (TCH) was observed to 10 % of WSF-AUCO. In oyster exposed to 20 % during 7 d significant decreases in immuno-hematological parameters associated with high lysosomal destabilization of hemocytes
and malondialdehyde (MDA) concentration was observed. The oysters showed compensatory cellular responses to the WSF-AUCO low concentrations, but these decreased during acute exposure. Immuno-modulator effects were induced by complex mixtures of compounds of WSF-AUCO. The molecular and immune-cellular responses estimated in P. imbricada offer appropriate information on the change in
the normal physiology of organisms that inhabit impacted environments by complex mixtures of xenobiotics.

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